Squabble

Sarek: "I don't want to hear it."
Spock: "But father...."
Sarek: "Talk to the hand, Spock."

Short Shot

Sisko: "Quick, Bashir! Shoot him!"
Bashir: "I'm trying, sir, but for some reason the phaser beam only shoots out two feet!"

Cloning Around

Picard: "Number One, you have the bridge until we can find out what to do about this cloning problem."

Pull!

Zek: "Here, boy, let me show you a little trick.  Pull my finger!  C'mon, pull it...pull it!"

Alternate ending for "Star Trek: Generations"

Riker: "I had always hoped I'd get a shot at this chair one day."
Picard: "Well, Will, it's your lucky day, because now it's all yours! Congratulations!"